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Putative sequence of Abeta(1-42) M35L

by James Stroud last modified 2008-09-04 06:32

Notes about the sequence of the Luhrs-Riek construct.

        10         20         30         40         50         60 
MRGSHHHHHH GSNANPNANP NANPNANPNA NPNANPNANP NANPNANPNA NPNANPNANP

70 80 90 100 110 120
NANPNANPNA NPNANPNANP NANPNANPRS MDAEFRHDSG YEVHHQKLVF FAEDVGSNKG

130
AIIGLLVGGV VIA

Parameters computed by ExPASy Protparam:

Number of amino acids: 133
Molecular weight: 13800.6
Theoretical pI: 6.74

Ext. coefficient: 1490 M-1 cm-1, at 280 nm

Full sequence without the numbering:

MRGSHHHHHHGSNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPRSMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLLVGGVVIA'

This is the non-Abeta leader from the above sequence:

MRGSHHHHHHGSNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPNANPRSM

These are 19 NANP repeats.

See: Proc Natl Acad Sci U S A. 2005 Nov 29;102(48):17342-7.

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